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2021b) (Yang et al

National survey of Japanese patients with mevalonate kinase deficiency reveals distinctive genetic and clinical characteristics

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The organic phase was evaporated and a solid was obtained (Scheme 17)

For cloning, the amplified products were purified using QIAquick PCR Purification kit (Qiagen Inc) and incubated at 72 C for 10 min in a reaction mixture (25 L) containing 200 M of dATP, 1.5 mM MgCl 2 , 0.625 unit of Taq DNA polymerase and 1X buffer, to add A-tail