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However, several drawbacks are associated with this technique, namely repeated monthly injections, poor patient compliance, and increased risks for endophthalmitis and retinal detachment [67, 68]

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In brief, tissue was fixed with either icecold acetone/methanol (1:1) for 1 min or 4% PFA for 2030 min followed by permeabilizing with 0.10.3% Triton X

With the help of the good model, particles from a total of 1210 micrographs were re-picked using Topaz 55 , and seed-facilitated 3D classification 56 was performed to improve resolution