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Species verification PCR amplification was performed using ITS2 forward primer (5-TGTGTCGATGAAGAGCGCAG) and ITS2 reverse primer (5-TGGTTAGTTTCTTTTCCTCCGC), as described previously (Morphew et al ., Reference Morphew, Eccleston, Wilkinson, McGarry, Perally, Prescott, Ward, Williams, Paterson, Raman and Ravikumar2012)

(3) Emerging techniques continue to discover novel modifications and crosstalk between different modifications, highlighting untapped research avenues

This strategy is more relevant for enhancing treatment with DNA-damaging chemotherapeutics than with targeted therapeutics, as the chemotherapeutic treatments generally lead to SASP-induction 47