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Description

Our review builds upon the 2023 findings by formally establishing this approach, offering a practical guide for clinicians treating newly diagnosed IGS patients

Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin