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According to one study [110], lung tissues from COPD patients exhibit ferroptosis due to hypermethylation of the CpG region of the Nrf2 promoter, which in turn prevents expression of Nrf2/GPX4

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However, it is limited by its qualitative or semi-quantitative nature, relatively low specificity, the need for precise primer design, and challenges arising from uneven 5mC distribution (62)