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Yes, L-glutamine degrades in complete media over time

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[DOI] [PMC free article] [PubMed] [Google Scholar] 31.Zhang L et al

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At the same time, the side effects caused by specific inhibition of glutamine synthetase are much smaller than those caused by glutamine analog therapy (84)

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680,681 This effect is mediated through the Raf/MAPK/ERK/c-Myc and PI3K/Akt pathways, which increase GLUT1 and HK expression

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