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HUVEC cells were stained with 0.5 M Calcein-AM and the fluorescence was detected by fluorescence microscope, and quantitative analysis of each image was performed using Image J 1.53t software

van der Merwe et al., 2015)

Thank you to our sponsors AG1 (Athletic Greens): InsideTracker: Thesis: Supplements from Momentous For the full show notes, visit hubermanlab.com Timestamps (00:00:00) Introducing Time Perception, Note on Fasting & Supplements (00:05:12) Sponsors (00:09:25) Entrainment, Circannual Entrainment, Melatonin (00:13:20) Seasonal Oscillations in Testosterone & Estrogen, Tool 1 (00:16:06) Circadian Timing, Tools 1, 2, 3 (for Circadian Entrainment) (00:21:13) Tool 4: Timing Physical Activity

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