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Reconstitution Troubleshooting Quality Verification Checklist Before using reconstituted IGF-1 LR3 in experiments: [ ] Confirm lot certificate of analysis (HPLC purity 98%, mass spec identity) [ ] Document reconstitution date, solvent, concentration, volume [ ] Visual inspection: solution is clear and colorless [ ] Aliquots labeled with compound, concentration, date, freeze-thaw count [ ] First-use aliquot shows expected biological activity in pilot assay For information on what certificates of analysis should contain, see: Quality Testing and Third-Party Verification for IGF-1 LR3 Research Peptides

Fifteen days after the treatment, all the mice were sacrificed and the tumorous tissue obtained (photographs of the tumor: Fig

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After incubation, the protein solution was analysed by gel filtration chromatography on a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare), which was connected to an KTAFPLC system (GE Healthcare)