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Effects of polysaccharides from Gastrodia elata on the immunomodulatory activity and gut microbiota regulation in cyclophosphamide-treated mice

After the washes, the brains were mounted in Vectashield (H-1000-10

Trends Plant Sci 11:329334 Doubnerov V, Rylav H (2011) What can enzymes of C4 photosynthesis do for C3 plants under stress

Recover and wash beads Elute proteins for SDS-PAGE analysis Analyze results In some steps, spin columns can be used to improve assay speed, protein recovery, reproducibility, and decrease elution volumes [2]