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Modern Pull-Down technology is not only limited to traditional affinity purification methods, but also developed a variety of variants, such as GST Pull-Down, His-tag Pull-Down, Biotin-Streptavidin Pull-Down, and magnetic bead-based co-immunoprecipitation (Co-IP), which has played an increasingly important role in proteomics, signal transduction, and protein modification research
Access this article Similar content being viewed by others Abbreviations AlaAT: Alanine aminotransferase AGAT II: Alanine-glyoxylate aminotransferase isoenzyme II BCAT c : Cytosolic branched-chain aminotransferase BCAT m : Mitochondrial branched-chain aminotransferase BCDHC: Branched-chain -keto acid dehydrogenase complex BTC: S -(2-Benzothiazolyl)-l-cysteine cAAT: Cytosolic aspartate aminotransferase DCVC: S -(1,2-Dichlorovinyl)-l-cysteine DFTAL: Difluorothioamidyl-lysine EDAG: -Glutamyldehydroalanylglycine ESI-MS: Electrospray ionization-mass spectrometry GSH: Glutathione GST: Glutathione S -transferase GTK: Glutamine transaminase K HSP60: Heat shock protein 60 kDa HSP70 i : Heat shock protein 70 kDa (inducible isoform) Hsc70: Cytosolic HSP70 KAT I: Kynurenine aminotransferase isoenzyme I KGDHC: -Ketoglutarate dehydrogenase complex mAAT: Mitochondrial aspartate aminotransferase mitACON: Mitochondrial aconitase mitHSP70: Heat shock protein 70 kDa (mitochondrial isoform) mitGTK: Mitochondrial GTK pmAAT: Precursor to mAAT PDHC: Pyruvate dehydrogenase complex PLP: Pyridoxal 5-phosphate PMP: Pyridoxamine 5-phosphate SAC: S -Allyl-l-cysteine SAMC: S -Allylmercapto-l-cysteine TCA cycle: Tricarboxylic acid cycle TFA: Trifluoroacetic acid TFEC: S -(1,1,2,2-Tetrafluoroethyl)-l-cysteine THT: Tetrahydrothiophene THT-A: -( S -Tetrahydrothiophenium)-l-alanine References Abraham DG, Cooper AJL (1991) Glutamine transaminase K and cysteine S -conjugate -lyase activity stains
Diagnosed B12 deficiency: Symptoms include fatigue, pale skin, shortness of breath, tingling in hands/feet, and mood changes
