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The RNAse, sodium hydroxide (NaOH), and hydrochloric acid (HCl) were purchased from VWR (Gdask, Poland)

Historically, a complex bidirectional communication system between the gastrointestinal tract, its microbiota, and the CNScomprising neural, hormonal, and immune pathwayshas been recognized and termed the MGB axis (51)

HVR: hypervariable region

Both the SWI/SNF inhibitor AU-15330 and the mitochondrial complex I oxidative phosphorylation inhibitor IACS-010759 showed pronounced synergy with bi-steric mTORC1 inhibitors to cause cuproptotic cell death in RP/MRD cells, suggesting these combinations as a potential patient treatment strategy for rapalog resistance