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typhimurium activates caspase-1/4/11-GSDMD inflammasome pathway, while acetaminophen toxicity begins with its metabolic conversion to reactive chemical species, N-acetyl-p-benzoquinoneimine (NAPQI), which causes hepatocyte necrosis 47

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Untargeted analysis of intracellular metabolites by UHPLC coupled to a Q-Exactive mass spectrometer (reversed-phase acetonitrile method) The profiling experiment was performed with a Dionex Ultimate 3000 UHPLC system (Thermo Scientific) coupled to a Q-Exactive (Thermo Scientific) equipped with an electrospray source operating in both positive and negative mode and full scan mode from 100 to 1,200 m/z