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Description

binding buffer: 10 mM TrisHCl, 154 mM NaCl, 1 mM CaCl 2 , 0.01% BSA, pH 7.4

When fasting, Sirt5 knockout mouse has reduced ECHA (an enzyme regulates the oxidation of fatty acid) activity, increased long-chain acyl-CoAs content and decreased ATP content

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It should be taken into account that carnitine synthesis is a reasonably slow process and does not readily keep up with fast changes of the metabolic requirements in stress conditions [14]