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Determination of glutathione S-transferase and glutathione reductase activity To obtain cellular extract we collected the cells with 25 mM glicil-glicine pH 7.4, 150 mM ClK, 5 mM MgSO 4 , 5 mM EDTA-Na 2 , 10 mM -mercaptoethanol

[PubMed ID: 39034407]

The differences are not likely to result from differences in antibody affinity, as all were at least one order of magnitude above the binding of p28 for either TAp63 or TAp73, and p28 immunoprecipitated a similar amount of p63 and p73 from the p53 mut MDA-MB-231 cell line

Quantification of ATM expression Cultured hair follicle melanocytes were seeded into 8-well chamber slides (~ 10,000 cells/well) and left to attach for at least 24 h, fixed in ice-cold methanol for 10 min, and incubated with blocking buffer of 10% donkey serum (Sigma-Aldrich, UK) for at least 30 min

Microsomes were prepared by sonication of 10 8 cells, removal of debris by centrifugation at 10,000 g for 15 min, and subsequent centrifugation of the resulting supernatant at 100,000 g for 60 min