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All RT-qPCR primers can be searched in support information (Table S1)

Furthermore, increased copper levels can increase reactive oxygen species (ROS) production and activate the NF-B signalling pathway, exacerbating inflammatory changes within the vascular wall and promoting atherosclerosis (25)

His plasma cysteine and plasma sulfate levels (measured by Great Smokies Lab) increased in the first 5 weeks from well below to almost into the bottom of the reference range