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The location of the ascorbate-binding site overlaps with the GSH-binding site, suggesting a ping-pong kinetic mechanism for electron transfer at the common Cys20 active site

Obesity and metabolic syndrome are associated with lower GSH-to-GSSG ratios indicating oxidative stress but this reflects increased demand rather than absolute deficiency

However, because dietary groups differed prior to intervention, the study cannot fully disentangle prebiotic effects from those attributable to the pre-existing diet

This NO-system interaction extends to blood pressure regulation, gastrointestinal motility, and wound healing

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