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Immunofluorescence staining Immunofluorescence staining was performed according to previous study [41]

Approaches based on the use of mass spectrometry for the identification of interacting proteins involved in iron trafficking have proven successful

Betz, V., & Rose, J

By leucocyte-depletion of RBC, by inhibiting proteases with phenylmethyl sulfonyl chloride (PMSF), by surface- 125 I -iodinating young and old RBC, by extraction with Triton and addition of a second antibody it was demonstrated that preexisting immune complexes contained band 3 monomers and oligomers in samples from old but not young RBC (Lutz and Stringaro-Wipf, 1983)