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Abbreviations: NEM, N-ethylmaleimide

MSM is just one way to obtain sulfur

A three-year trial of DHA supplementation (800 mg/day) in elderly adults without dementia is currently under way (see www.clinicaltrials.gov)

Gently inject the solution into the vial of lyophilized powder, angling the needle so the liquid runs down the inside wall of the vial

Clarified lysate was next applied to a Ni Sepharose 6 FF column (Cytiva) linked to an AKTA Avant150 FPLC system for immobilized metal affinity chromatography