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Cells were cultured and maintained DMEM/Ham's F-12 (Merck KGaA) supplemented with 10% FBS (Thermo Fisher Scientific, Inc.) and 100 g/ml streptomycin/100 units/ml penicillin (Thermo Fisher Scientific, Inc.) at 37C in a humidified atmosphere containing 5% CO2

doi: 10.1111/cns.2012.18.issue-12 294 LuSMaZGuYLiPChenYBaiMet al

At lower concentrations of APE (62.5 and 125 g/mL), a modest decline in NO levels was observed, while higher concentrations (250, 500, 1000, and 2000 g/mL) exhibited progressively stronger suppression of NO production, greater than 50%

Yuichiro Yamada at Akita University for generously donating the tagged human GLP-1R cytomegalovirus promoter vector (pCMV)

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