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Precipitated cytosolic and GST samples were then resolubilized in isoelectric focusing buffer containing 8 M Urea, 2% w/v CHAPS, 33 mm DTT and 0.5% v/v ampholytes (pH 310)

Phase I reactions may not necessarily generate a pharmacologically inactive species, but Phase II reactions are regarded as detoxifying and will inactivate drugs to prevent damage to macromolecules or to prepare drugs for efficient elimination

To overcome this problem, z-stacks (series of focal planes on the z axis) collected during image acquisition are then processed using image deconvolution software

J Pharmacol Exp Ther 322(1):108116 Flora G, Gupta D, Tiwari A (2012) Toxicity of lead: a review with recent updates