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Whole blood was collected from mice by cardiac puncture in BD vacutainer tubes (Fisher Scientific, Hampton, NH, USA) and centrifuged for 40 min at 2000 revolutions per minute to separate serum

126 SalasA.Hernandez-RochaC.DHaensG.DuijvesteinM.FaubionW.McGovernD.et al

J Nutr Biochem 7:6676

Most patients tolerate this without issue, but if you go into surgery with a reduced red cell count, your oxygen-carrying capacity is already compromised before the incision is made