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The secondary antibodies used were: anti-rabbit IgG Alexa 488 conjugated (1:500, A-21206, Thermo Fisher), anti-rat IgG Alexa 488 conjugated (1:500, A-21208, Thermo Fisher), anti-mouse IgG Alexa 488 conjugated (1:500, A-28175, Thermo Fisher), anti-rabbit IgG Alexa 594 conjugated (1:500, A-11037, Thermo Fisher), anti-mouse IgG Alexa 594 conjugated (1:500, A-11032, Thermo Fisher), anti-guinea pig IgG Alexa 594 conjugated (1:500, A-11076, Thermo Fisher), anti-chicken IgY Alexa 647 conjugated (1:500, A-21449, Thermo Fisher), anti-rat IgG Alexa 647 conjugated (1:500, A-21247, Thermo Fisher) and anti-mouse IgG Alexa 647 conjugated (1:500, A-28181, Thermo Fisher)