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Primary human hepatocytes were exposed for 24 h to either DMSO (control) or to increasing concentrations of PMR extract in 6-well culture plates

24 h before the analysis, cells were serum starved in +Ser +Gly or -Ser -Gly media

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Sharmila G, Nikitha VS, Ilaiyarasi S, Dhivya K, Rajasekar V, Kumar ANM, et al

Consistently, another study demonstrated that abnormal PKM2/PKM1 alternative splicing results in enhanced PKM1 and diminished PKM2 mRNA expression, attenuates decidualization, and may contributed to repeated implantation failure (41) ( Figure 1 )