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Finally, when the IMPACT-CN system is used for C-terminal intein cleaving, a small peptide of about 1.2 kDa cleaves from the intein tag and elutes alongside the POI, requiring removal by dialysis ( The Second-Generation Patents Tried to Come up With Better Intein and Cloning Systems The patents that followed the original NEB patents describe better contiguous inteins with more controllable and efficient cleaving properties