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Lethal fraction scores were calculated at each time point as previously described, with two modifications (Forcina et al, 2017): (i) because long-dead cells can release SG, the maximum number of SG positive counts from the start of the time course was used in these calculations

Aliquoting for extended storage Some researchers aliquot their reconstituted solution into single-use portions to minimize the number of times they pierce the vial stopper