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The western blots were incubated with HRP-labeled secondary antibody and the protein bands were developed using Lumi-Light Plus chemiluminescent reagent (Roche, Indianapolis, IN)

Hedman CA, Frystyk J, Lindstrm T, Oskarsson P, Arnqvist HJ

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171 The succinate receptor (SUCNR1) is highly expressed on human mast cells, and pretreatment with succinate enhances IgEmediated human mast cell activation and antigeninduced airway contraction