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After the 24-hours treatment, the top layer of tissue models are further treated with 200 l of methanol for 2 min at room temperature and, after that, they are rinsed twice with 200 l of phosphate-buffered saline (PBS, Sigma)

The expression of MiR-335 is modulated by lipid loading, resulting in overexpression in liver and adipose tissue of obese mice 93

T., Hwu, W

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This study utilized a randomized, placebo-controlled, double-blind, multicenter study design, a full 10-week supplementation, blood monitoring at baseline and posttreatment, and multiple assessments of various functions