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Protein expression The DNA sequence of the NTD sequence of CSP, residues 17-104, was amplified by PCR using sequence specific primers (NTD-GST-F: TGGGATCCAGGAGGCCTTATTCCAGGAA and NTD-GST-R: CACTCGAGCTA TGGATCTACATTTGGGTTGGCAT) with BamHI and XhoI restriction sites, respectively

Extended contact time with the sublingual membrane maximizes absorption opportunity

Plants are generally not adequate sources of B12

Statistical analyses were performed using GraphPad Prism software (version 8.0)

GSNO is metabolized by the NADH-dependent GSNOR, which thereby shifts the cellular equilibrium to disfavor protein SNOs, while Trxs and TrxRs denitrosylate proteins directly