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Brain tissues were placed in PBS for 24 hours before being embedded in paraffin wax and sectioned onto histology slides

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MTT 1-(4,5-Dimethylthiazol-2-yl) 3,5-diphenylformazan reagent HGF-dependent proliferation was determined by subtracting the basal proliferation (in the absence of HGF) from total proliferation rates in groups containing HGF